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KSR2 overexpression associated with an immunosuppressive tumor microenvironment. A Flow cytometry analysis of tumor-infiltrating CD4⁺ T, CD8⁺ T, and regulatory T cells ( n = 3 mice). B , C ELISA quantification of GzmB ( B ) and IFN-γ ( C ) levels ( n = 3 mice). D MIF analysis (CD8, red; GzmB, green; Treg, yellow; DAPI, blue) and cell quantification (3 fields/sample; n = 3 mice). Mean ± SD. two-tailed unpaired t test or Welch’s t test (* P < 0.05, ** P < 0.01, *** P < 0.001)

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: KSR2 functions as a metabolic checkpoint for anti-PD-1 resistance by reprogramming glucose metabolism

doi: 10.1007/s00262-026-04394-z

Figure Lengend Snippet: KSR2 overexpression associated with an immunosuppressive tumor microenvironment. A Flow cytometry analysis of tumor-infiltrating CD4⁺ T, CD8⁺ T, and regulatory T cells ( n = 3 mice). B , C ELISA quantification of GzmB ( B ) and IFN-γ ( C ) levels ( n = 3 mice). D MIF analysis (CD8, red; GzmB, green; Treg, yellow; DAPI, blue) and cell quantification (3 fields/sample; n = 3 mice). Mean ± SD. two-tailed unpaired t test or Welch’s t test (* P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Mouse tumor homogenates were prepared, and concentrations of granzyme B and IFN-γ were measured using the mouse granzyme B ELISA kit (E-EL-M0594, Elabscience) and mouse IFN-γ ELISA kit (E-HSEL-M0007, Elabscience), respectively, according to the manufacturers’ protocols.

Techniques: Over Expression, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Two Tailed Test

KSR2 knockdown reverses metabolic reprogramming and reinstates anti-tumor immunity. A Differential metabolite abundance in control versus Ksr2-knockdown tumors ( n = 3 mice). B IHC analysis of LDHA and HK2 expression. 3 fields/sample; n = 3 mice. Scale bars, 100μm, 20 μ m. C , D GzmB ( C ) and IFN-γ ( D ) levels in tumors by ELISA ( n = 3). E MIF analysis and cell quantification (3 fields/sample; n = 3 mice). Mean ± SD. Two-tailed unpaired t test or Welch’s t test (* P < 0.05, ** P < 0.01, *** P < 0.001)

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: KSR2 functions as a metabolic checkpoint for anti-PD-1 resistance by reprogramming glucose metabolism

doi: 10.1007/s00262-026-04394-z

Figure Lengend Snippet: KSR2 knockdown reverses metabolic reprogramming and reinstates anti-tumor immunity. A Differential metabolite abundance in control versus Ksr2-knockdown tumors ( n = 3 mice). B IHC analysis of LDHA and HK2 expression. 3 fields/sample; n = 3 mice. Scale bars, 100μm, 20 μ m. C , D GzmB ( C ) and IFN-γ ( D ) levels in tumors by ELISA ( n = 3). E MIF analysis and cell quantification (3 fields/sample; n = 3 mice). Mean ± SD. Two-tailed unpaired t test or Welch’s t test (* P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Mouse tumor homogenates were prepared, and concentrations of granzyme B and IFN-γ were measured using the mouse granzyme B ELISA kit (E-EL-M0594, Elabscience) and mouse IFN-γ ELISA kit (E-HSEL-M0007, Elabscience), respectively, according to the manufacturers’ protocols.

Techniques: Knockdown, Control, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test